Molecular characterization of spontaneous mesenchymal stem cell transformation.

ABSTRACT:

Background

We previously reported the in vitro spontaneous transformation of human mesenchymal stem cells (MSC) generating a population with tumorigenic potential, that we termed transformed mesenchymal cells (TMC).

Methodology/principal findings

Here we have characterized the molecular changes associated with TMC generation. Using microarrays techniques we identified a set of altered pathways and a greater number of downregulated than upregulated genes during MSC transformation, in part due to the expression of many untranslated RNAs in MSC. Microarray results were validated by qRT-PCR and protein detection.

Conclusions/significance

In our model, the transformation process takes place through two sequential steps; first MSC bypass senescence by upregulating c-myc and

SUBMITTER: Rubio D 

PROVIDER: S-EPMC2151133 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature