Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes.

ABSTRACT: Accurate determination of the relative positions of proteins within localized regions of the cell is essential for understanding their biological function. Although fluorescent fusion proteins are targeted with molecular precision, the position of these genetically expressed reporters is usually known only to the resolution of conventional optics ( approximately 200 nm). Here, we report the use of two-color photoactivated localization microscopy (PALM) to determine the ultrastructural relationship between different proteins fused to spectrally distinct photoactivatable fluorescent proteins (PA-FPs). The nonperturbative incorporation of these endogenous tags facilitates an imaging resolution in whole, fixed cells of approximately 20-30 nm at acquisition times of 5-30 min. We apply the techn

SUBMITTER: Shroff H 

PROVIDER: S-EPMC2154427 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature