A coactivator trap identifies NONO (p54nrb) as a component of the cAMP-signaling pathway.

ABSTRACT: Signal transduction pathways often use a transcriptional component to mediate adaptive cellular responses. Coactivator proteins function prominently in these pathways as the conduit to the basic transcriptional machinery. Here we present a high-throughput cell-based screening strategy, termed the "coactivator trap," to study the functional interactions of coactivators with transcription factors. We applied this strategy to the cAMP signaling pathway, which utilizes two families of coactivators, the cAMP response element binding protein (CREB) binding protein (CBP)/p300 family and the recently identified transducers of regulated CREB activity family (TORCs1-3). In addition to identifying numerous known interactions of these coactivators, this analysis identified NONO (p54(nrb)) as a TORC-in

SUBMITTER: Amelio AL 

PROVIDER: S-EPMC2154428 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature