Oncogenic H-Ras V12 promotes anchorage-independent cytokinesis in human fibroblasts.

ABSTRACT: Cell anchorage is required for cell proliferation of untransformed cells, whereas anchorage-independent growth can be induced by oncogenes and is a hallmark of transformation. Whereas anchorage-dependent control of the progression of the G(1) phase of the cell cycle has been extensively studied, it is less clear whether and how anchorage may control other cell cycle phases and whether oncogenes may affect such controls. Here, we found that lack of cell anchorage did not influence progression through the cell cycle S phase, G(2) phase, or most of mitosis of primary human fibroblasts. However, unanchored fibroblasts could not complete cytokinesis. The cleavage furrow and central spindle were still formed in the absence of anchorage, but cells were unable to complete ingression, causing binuc

SUBMITTER: Thullberg M 

PROVIDER: S-EPMC2154432 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature