Physiological and transcriptional responses of Saccharomyces cerevisiae to zinc limitation in chemostat cultures.

ABSTRACT: Transcriptional responses of the yeast Saccharomyces cerevisiae to Zn availability were investigated at a fixed specific growth rate under limiting and abundant Zn concentrations in chemostat culture. To investigate the context dependency of this transcriptional response and eliminate growth rate-dependent variations in transcription, yeast was grown under several chemostat regimens, resulting in various carbon (glucose), nitrogen (ammonium), zinc, and oxygen supplies. A robust set of genes that responded consistently to Zn limitation was identified, and the set enabled the definition of the Zn-specific Zap1p regulon, comprised of 26 genes and characterized by a broader zinc-responsive element consensus (MHHAACCBYNMRGGT) than so far described. Most surprising was the Zn-dependent regulatio

SUBMITTER: De Nicola R 

PROVIDER: S-EPMC2168061 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature