Evaluation of a multiplexed PCR assay for detection of respiratory viral pathogens in a public health laboratory setting.

ABSTRACT: There are numerous viral and bacterial causes of respiratory disease. To enable rapid and sensitive detection of even the most prevalent causes, there is a need for more-simplified testing systems that enable researchers and clinicians to perform multiplexed molecular diagnostics quickly and easily. To this end, a new multiplexed molecular test called the MultiCode-PLx respiratory virus panel (PLx-RVP) was developed and then implemented in a public health laboratory setting. A total of 687 respiratory samples were analyzed for the presence of 17 viruses that commonly cause respiratory disease. As a comparator, the samples were also tested using a standard testing algorithm that included the use of a real-time influenza virus A and B reverse transcription-PCR test and routine viral culture

SUBMITTER: Marshall DJ 

PROVIDER: S-EPMC2168541 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature