Gene expression of commensal Lactobacillus johnsonii strain NCC533 during in vitro growth and in the murine gut.

ABSTRACT: Work with pathogens like Vibrio cholerae has shown major differences between genes expressed in bacteria grown in vitro and in vivo. To explore this subject for commensals, we investigated the transcription of the Lactobacillus johnsonii NCC533 genome during in vitro and in vivo growth using the microarray technology. During broth growth, 537, 626, and 277 of the 1,756 tested genes were expressed during exponential phase, "adaptation" (early stationary phase), and stationary phase, respectively. One hundred one, 150, and 33 genes, respectively, were specifically transcribed in these three phases. To explore the in vivo transcription program, we fed L. johnsonii containing a resistance plasmid to antibiotic-treated mice. After a 2-day washout phase, we determined the viable-cell counts of l

SUBMITTER: Denou E 

PROVIDER: S-EPMC2168692 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature