Corynebacterium diphtheriae: identification and characterization of a channel-forming protein in the cell wall.

ABSTRACT: The cell wall fraction of the gram-positive, nontoxic Corynebacterium diphtheriae strain C8r(-) Tox- (=ATCC 11913) contained a channel-forming protein, as judged from reconstitution experiments with artificial lipid bilayer experiments. The channel-forming protein was present in detergent-treated cell walls and in extracts of whole cells obtained using organic solvents. The protein had an apparent molecular mass of about 66 kDa as determined on Tricine-containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and consisted of subunits having a molecular mass of about 5 kDa. Single-channel experiments with the purified protein suggested that the protein formed channels with a single-channel conductance of 2.25 nS in 1 M KCl. Further single-channel analysis suggested that the

SUBMITTER: Schiffler B 

PROVIDER: S-EPMC2168714 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature