Mapping protease inhibitor resistance to human immunodeficiency virus type 1 sequence polymorphisms within patients.

ABSTRACT: Resistance genotyping provides an important resource for the clinical management of patients infected with human immunodeficiency virus type 1 (HIV-1). However, resistance to protease (PR) inhibitors (PIs) is a complex phenotype shaped by interactions among nearly half of the residues in HIV-1 PR. Previous studies of the genetic basis of PI resistance focused on fixed substitutions among populations of HIV-1, i.e., host-specific adaptations. Consequently, they are susceptible to a high false discovery rate due to founder effects. Here, we employ sequencing "mixtures" (i.e., ambiguous base calls) as a site-specific marker of genetic variation within patients that is independent of the phylogeny. We demonstrate that the transient response to selection by PIs is manifested as an excess of non

SUBMITTER: Poon AF 

PROVIDER: S-EPMC2168824 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature