Transcription alters chromosomal locations of cohesin in Saccharomyces cerevisiae.

ABSTRACT: In eukaryotic cells, cohesion between sister chromatids allows chromosomes to biorient on the metaphase plate and holds them together until they separate into daughter cells during mitosis. Cohesion is mediated by the cohesin protein complex. Although the association of this complex with particular regions of the genome is highly reproducible, it is unclear what distinguishes a chromosomal region for cohesin association. Since one of the primary locations of cohesin is intergenic regions between converging transcription units, we explored the relationship between transcription and cohesin localization. Chromatin immunoprecipitation followed by hybridization to a microarray (ChIP chip) indicated that transcript elongation into cohesin association sites results in the local disassociation of

SUBMITTER: Bausch C 

PROVIDER: S-EPMC2169412 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature