MukB colocalizes with the oriC region and is required for organization of the two Escherichia coli chromosome arms into separate cell halves.

ABSTRACT: The circular Escherichia coli chromosome is organized by bidirectional replication into two equal left and right arms (replichores). Each arm occupies a separate cell half, with the origin of replication (oriC) at mid-cell. E. coli MukBEF belongs to the ubiquitous family of SMC protein complexes that play key roles in chromosome organization and processing. In mukBEF mutants, viability is restricted to low temperature with production of anucleate cells, reflecting chromosome segregation defects. We show that in mukB mutant cells, the two chromosome arms do not separate into distinct cell halves, but extend from pole to pole with the oriC region located at the old pole. Mutations in topA, encoding topoisomerase I, do not suppress the aberrant positioning of chromosomal loci in mukB cells, d

SUBMITTER: Danilova O 

PROVIDER: S-EPMC2169520 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature