Microtubules are the only structural constituent of the spindle apparatus required for induction of cell cleavage.

ABSTRACT: Structural constituents of the spindle apparatus essential for cleavage induction remain undefined. Findings from various cell types using different approaches suggest the importance of all structural constituents, including asters, the central spindle, and chromosomes. In this study, we systematically dissected the role of each constituent in cleavage induction in grasshopper spermatocytes and narrowed the essential one down to bundled microtubules. Using micromanipulation, we produced "cells" containing only asters, a truncated central spindle lacking both asters and chromosomes, or microtubules alone. We show that furrow induction occurs under all circumstances, so long as sufficient microtubules are present. Microtubules, as the only spindle structural constituent, undergo dramatic, st

SUBMITTER: Alsop GB 

PROVIDER: S-EPMC2172685 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature