Roles of fission yeast tea1p in the localization of polarity factors and in organizing the microtubular cytoskeleton.

ABSTRACT: The cylindrical shape of the fission yeast cell is generated by linear polarized growth from its cell ends. Using immunofluorescence and live imaging microscopy, we have investigated the roles of the cell end marker tea1p in generating linear polarized growth. We found that tea1p is primarily transported on plus ends of microtubules from the vicinity of the nucleus to the cell ends, and that its movement near the nucleus is independent of the kinesin tea2p. Deletion analysis identified a coiled-coil domain in tea1p essential for its retention at cell ends, and demonstrated that tea1p exerts different functions dependent on its location. On the tips of microtubules, tea1p prevents the curling of microtubules around the cell ends, whereas it is required for maintaining linear cell growth and

SUBMITTER: Behrens R 

PROVIDER: S-EPMC2173414 | biostudies-literature | 2002 May

REPOSITORIES: biostudies-literature