Defective flagellar assembly and length regulation in LF3 null mutants in Chlamydomonas.

ABSTRACT: Four long-flagella (LF) genes are important for flagellar length control in Chlamydomonas reinhardtii. Here, we characterize two new null lf3 mutants whose phenotypes are different from previously identified lf3 mutants. These null mutants have unequal-length flagella that assemble more slowly than wild-type flagella, though their flagella can also reach abnormally long lengths. Prominent bulges are found at the distal ends of short, long, and regenerating flagella of these mutants. Analysis of the flagella by electron and immunofluorescence microscopy and by Western blots revealed that the bulges contain intraflagellar transport complexes, a defect reported previously (for review see Cole, D.G., 2003. Traffic. 4:435-442) in a subset of mutants defective in intraflagellar transport. We hav

SUBMITTER: Tam LW 

PROVIDER: S-EPMC2173655 | biostudies-literature | 2003 Nov

REPOSITORIES: biostudies-literature