Distinct pathways for the early recruitment of myosin II and actin to the cytokinetic furrow.

ABSTRACT: Equatorial organization of myosin II and actin has been recognized as a universal event in cytokinesis of animal cells. Current models for the formation of equatorial cortex favor either directional cortical transport toward the equator or localized de novo assembly. However, this process has never been analyzed directly in dividing mammalian cells at a high resolution. Here we applied total internal reflection fluorescence microscope (TIRF-M), coupled with spatial temporal image correlation spectroscopy (STICS) and a new analytical approach termed temporal differential microscopy (TDM), to image the dynamics of myosin II and actin during the assembly of equatorial cortex. Our results indicated distinct and at least partially independent mechanisms for the early equatorial recruitment of m

SUBMITTER: Zhou M 

PROVIDER: S-EPMC2174197 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature