Pan LF-ELISA using BmR1 and BmSXP recombinant antigens for detection of lymphatic filariasis.

ABSTRACT:

Background

Anti-filarial IgG4 antibody has been shown to be a good marker for detection of lymphatic filaria infection. Previous studies demonstrated that anti-filarial IgG4 assay using BmR1 recombinant antigen was highly specific and sensitive for detection of brugian filariasis. For bancroftian filariasis, an equivalent assay employing recombinant antigen expressed from the ORF of SXP1 gene has been reported. In order to detect infections by all species of lymphatic filarial, BmR1 and BmSXP recombinant antigens were employed in the development of a pan LF-ELISA.

Methods

BmR1 was previously produced while BmSXP recombinant antigen was produced by cloning the ORF of SXP1 gene from a Brugia malayi cDNA library, followed by expression in a bacterial expression system. Subseque

SUBMITTER: Abdul Rahman R 

PROVIDER: S-EPMC2174454 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature