Role of adipocyte-derived lipoprotein lipase in adipocyte hypertrophy.

ABSTRACT:

Background

A major portion of available fatty acids for adipocyte uptake is derived from lipoprotein lipase (LPL)-mediated hydrolysis of circulating lipoprotein particles. In vivo studies aimed at identifying the precise role of adipocyte-derived LPL in fat storage function of adipose tissue have been unable to provide conclusive evidence due to compensatory mechanisms that activate endogenous fatty acid synthesis. To address this gap in knowledge, we have measured the effect of reducing adipocyte LPL expression on intracellular lipid accumulation using a well-established cultured model of adipocyte differentiation.

Methods

siRNA specific for mouse LPL was transfected into 3T3-L1 adipocytes. Expression of LPL was measured by quantitative real-time PCR and cell surface-associ

SUBMITTER: Gonzales AM 

PROVIDER: S-EPMC2174487 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature