Sex specific gene regulation and expression QTLs in mouse macrophages from a strain intercross.

ABSTRACT:

Background

A powerful way to identify genes for complex traits it to combine genetic and genomic methods. Many trait quantitative trait loci (QTLs) for complex traits are sex specific, but the reason for this is not well understood.

Methodology/principal findings

RNA was prepared from bone marrow derived macrophages of 93 female and 114 male F(2) mice derived from a strain intercross between apoE-deficient mice on the AKR and DBA/2 genetic backgrounds, and was subjected to transcriptome profiling using microarrays. A high density genome scan was performed using a mouse SNP chip, and expression QTLs (eQTLs) were located for expressed transcripts. Using suggestive and significant LOD score cutoffs of 3.0 and 4.3, respectively, thousands of eQTLs in the female and male cohorts

SUBMITTER: Bhasin JM 

PROVIDER: S-EPMC2174529 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature