Transcriptional regulation of human eosinophil RNases by an evolutionary- conserved sequence motif in primate genome.

ABSTRACT:

Background

Human eosinophil-derived neurotoxin (edn) and eosinophil cationic protein (ecp) are members of a subfamily of primate ribonuclease (rnase) genes. Although they are generated by gene duplication event, distinct edn and ecp expression profile in various tissues have been reported.

Results

In this study, we obtained the upstream promoter sequences of several representative primate eosinophil rnases. Bioinformatic analysis revealed the presence of a shared 34-nucleotide (nt) sequence stretch located at -81 to -48 in all edn promoters and macaque ecp promoter. Such a unique sequence motif constituted a region essential for transactivation of human edn in hepatocellular carcinoma cells. Gel electrophoretic mobility shift assay, transient transfection and scanning mutage

SUBMITTER: Wang HY 

PROVIDER: S-EPMC2174947 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature