New transposon delivery plasmids for insertional mutagenesis in Bacillus anthracis.

ABSTRACT: Two new transposon delivery vector systems utilizing Mariner and mini-Tn10 transposons have been developed for in vivo insertional mutagenesis in Bacillus anthracis and other compatible Gram-positive species. The utility of both systems was directly demonstrated through the mutagenesis of a widely used B. anthracis strain.

SUBMITTER: Wilson AC 

PROVIDER: S-EPMC2175031 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature