Conserved nucleotides in an RNA essential for hepatitis B virus replication show distinct mobility patterns.

ABSTRACT: The number of regulatory RNAs with identified non-canonical structures is increasing, and structural transitions often play a role in their biological function. This stimulates interest in internal motions of RNA, which can underlie structural transitions. Heteronuclear NMR relaxation measurements, which are commonly used to study internal motion, only report on local motions of few sites within the molecule. Here we have studied a 27-nt segment of the human hepatitis B virus (HBV) pregenomic RNA, which is essential for viral replication. We combined heteronuclear relaxation with the new off-resonance ROESY technique, which reports on internal motions of H,H contacts. Using off-resonance ROESY, we could for the first time detect motion of through-space H,H contacts, such as in intra-residu

SUBMITTER: Petzold K 

PROVIDER: S-EPMC2175316 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature