Transcriptional networks in plasmacytoid dendritic cells stimulated with synthetic TLR 7 agonists.

ABSTRACT:

Background

Plasmacytoid Dendritic Cells (pDC) comprise approximately 0.2 to 0.8% of the blood mononuclear cells and are the primary type 1 interferon (IFN), producing cells, secreting high levels of IFN in response to viral infections. Plasmacytoid dendritic cells express predominantly TLRs 7 & 9, making them responsive to ssRNA and CpG DNA. The objective of this study was to evaluate the molecular and cellular processes altered upon stimulation of pDC with synthetic TLR 7 and TLR 7/8 agonists. To this end, we evaluated changes in global gene expression upon stimulation of 99.9% pure human pDC with the TLR7 selective agonists 3M-852A, and the TLR7/8 agonist 3M-011.

Results

Global gene expression was evaluated using the Affymetrix U133A GeneChip(R) and selected genes were con

SUBMITTER: Birmachu W 

PROVIDER: S-EPMC2175514 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature