Translation initiation region sequence preferences in Escherichia coli.

ABSTRACT:

Background

The mRNA translation initiation region (TIR) comprises the initiator codon, Shine-Dalgarno (SD) sequence and translational enhancers. Probably the most abundant class of enhancers contains A/U-rich sequences. We have tested the influence of SD sequence length and the presence of enhancers on the efficiency of translation initiation.

Results

We found that during bacterial growth at 37 degrees C, a six-nucleotide SD (AGGAGG) is more efficient than shorter or longer sequences. The A/U-rich enhancer contributes strongly to the efficiency of initiation, having the greatest stimulatory effect in the exponential growth phase of the bacteria. The SD sequences and the A/U-rich enhancer stimulate translation co-operatively: strong SDs are stimulated by the enhancer much mor

SUBMITTER: Vimberg V 

PROVIDER: S-EPMC2176067 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature