Secreted phospholipase A2 group X overexpression in asthma and bronchial hyperresponsiveness.

ABSTRACT:

Rationale

Secreted phospholipase A(2) enzymes (sPLA(2)s) play key regulatory roles in the biosynthesis of eicosanoids, such as the cysteinyl leukotrienes, but the role of these enzymes in the pathogenesis of asthma is not known.

Objectives

To establish if sPLA(2)s are overexpressed in the airways of patients with asthma, and to determine if these enzymes may play a role in the generation of eicosanoids in exercise-induced bronchoconstriction.

Methods

Induced sputum samples were obtained from subjects with asthma with exercise-induced bronchoconstriction and nonasthmatic control subjects at baseline, and on a separate day 30 minutes after exercise challenge. The expression of the PLA(2)s in induced sputum cells and supernatant was determined by quantitative polymerase

SUBMITTER: Hallstrand TS 

PROVIDER: S-EPMC2176098 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature