Variable prostaglandin E2 resistance in fibroblasts from patients with usual interstitial pneumonia.

ABSTRACT:

Rationale

Prostaglandin (PG) E2, a cyclooxygenase-derived lipid mediator, is a potent down-regulator of fibroblast activation in normal lung fibroblasts. Although fibroblasts from patients with idiopathic pulmonary fibrosis are known to exhibit a defect in PGE2 synthesis, there is little information about their responsiveness to this lipid mediator.

Objectives

To compare responses to PGE2 in normal, usual interstitial pneumonia (UIP), and other diffuse parenchymal lung disease (DPLD) fibroblasts.

Methods

Fibroblasts were grown in vitro from well characterized control (n = 7), UIP (n = 17), or other DPLD (n = 13) lung tissue. The effects of PGE2 on fibroblast proliferation and collagen expression were determined.

Measurements and main results

Only 3 of 12 UIP fi

SUBMITTER: Huang SK 

PROVIDER: S-EPMC2176116 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature