Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection.

ABSTRACT:

Background

Paracoccidioides brasiliensis ecology is not completely understood, although several pieces of evidence point to the soil as its most probable habitat. The present study aimed to investigate the fungal growth, conidia production and molecular pathogen detection in different soil conditions.

Methods

Soils samples of clayey, sandy and medium textures were collected from ground surface and the interior of armadillo burrows in a hyperendemic area of Paracoccidioidomycosis. P. brasiliensis was inoculated in soil with controlled humidity and in culture medium containing soil extracts. The molecular detection was carried out by Nested PCR, using panfungal and species specific primers from the ITS-5.8S rDNA region.

Results

The soil texture does not affect fungus de

SUBMITTER: Tercarioli GR 

PROVIDER: S-EPMC2180180 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature