Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments.

ABSTRACT: Compartmentalization of the nucleus is now recognized as an important level of regulation influencing specific nuclear processes. The mechanism of factor organization and the movement of factors in nuclear space have not been fully determined. Splicing factors, for example, have been shown to move in a directed manner as large intact structures from sites of concentration to sites of active transcription, but splicing factors are also thought to exist in a freely diffusible state. In this study, we examined the movement of a splicing factor, ASF, green fluorescent fusion protein (ASF-GFP) using time-lapse microscopy and the technique fluorescence recovery after photobleaching (FRAP). We find that ASF-GFP moves at rates up to 100 times slower than free diffusion when it is associated with s

SUBMITTER: Kruhlak MJ 

PROVIDER: S-EPMC2185567 | biostudies-literature | 2000 Jul

REPOSITORIES: biostudies-literature