Genome-scale functional profiling of the mammalian AP-1 signaling pathway.

ABSTRACT: Large-scale functional genomics approaches are fundamental to the characterization of mammalian transcriptomes annotated by genome sequencing projects. Although current high-throughput strategies systematically survey either transcriptional or biochemical networks, analogous genome-scale investigations that analyze gene function in mammalian cells have yet to be fully realized. Through transient overexpression analysis, we describe the parallel interrogation of approximately 20,000 sequence annotated genes in cancer-related signaling pathways. For experimental validation of these genome data, we apply an integrative strategy to characterize previously unreported effectors of activator protein-1 (AP-1) mediated growth and mitogenic response pathways. These studies identify the ADP-ribosylat

SUBMITTER: Chanda SK 

PROVIDER: S-EPMC218728 | biostudies-literature | 2003 Oct

REPOSITORIES: biostudies-literature