Comparative structural analysis of transcriptionally competent and incompetent rotavirus-antibody complexes.

ABSTRACT: During genome transcription in rotavirus, as with many segmented double-stranded RNA viruses, mRNA is transcribed within the intact subviral particle and translocated through specific channels in the capsid. To understand how the conformation of the capsid affects the efficiency of transcriptional events in the viral core, we carried out a series of comparative structural and biochemical studies to characterize four different structural forms of the virus exhibiting differing transcriptional behavior. Two of these were virus-antibody complexes having contrasting transcriptional capabilities, and two were variant structural forms of the virus that exist during the life cycle and also exhibit contrasting transcriptional behavior. Three-dimensional structural studies using electron cryomicros

SUBMITTER: Lawton JA 

PROVIDER: S-EPMC21876 | biostudies-literature | 1999 May

REPOSITORIES: biostudies-literature