Statins induce S1P1 receptors and enhance endothelial nitric oxide production in response to high-density lipoproteins.

ABSTRACT:

Background and purpose

Sphingosine 1-phosphate (S1P) is a serum-borne naturally occurring sphingolipid, specifically enriched in high-density lipoprotein (HDL) fractions. S1P binds to G-protein-coupled S1P1 receptors to activate endothelial NO synthase (eNOS) in vascular endothelial cells. We explored whether and how statins, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, modulate expression of S1P1 receptors and endothelial responses for subsequent stimulation with S1P or with HDL.

Experimental approach

Protein expression and phosphorylation and mRNA expression in cultured bovine aortic endothelial cells (BAEC) were determined using immunoblots and reverse transcription PCR analyses, respectively. NO synthesis was assessed as nitrite production.

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SUBMITTER: Igarashi J 

PROVIDER: S-EPMC2189725 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature