Evidence for the expression of multiple uracil nucleotide-stimulated P2 receptors coupled to smooth muscle contraction in porcine isolated arteries.

ABSTRACT:

Background and purpose

The uracil nucleotides UDP and UTP have been reported to activate P2Y2, P2Y4 and P2Y6 receptors to cause vasoconstriction. We have performed a comparative analysis of these receptors in endothelium-denuded smooth muscle from porcine isolated coronary and ear arteries, using pharmacological and molecular tools.

Experimental approach

Tissue segments were used to construct non-cumulative concentration response curves for UTP and UDP, in the absence and presence of the P2 receptor antagonists PPADS or suramin. RT-PCR and immunoblot analyses were employed to define gene expression and immunoreactivity for P2Y2, P2Y4 and P2Y6 receptors.

Key results

In the coronary artery, UTP-evoked contractile responses were reduced in the presence of suramin, but no

SUBMITTER: Rayment SJ 

PROVIDER: S-EPMC2189772 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature