Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration.

ABSTRACT: Integrins have been implicated in key cellular functions, including cytoskeletal organization, motility, growth, survival, and control of gene expression. The plethora of integrin alpha and beta subunits suggests that individual integrins have unique biological roles, implying specific molecular connections between integrins and intracellular signaling or regulatory pathways. Here, we have used a yeast two-hybrid screen to identify a novel protein, termed Nischarin, that binds preferentially to the cytoplasmic domain of the integrin alpha5 subunit, inhibits cell motility, and alters actin filament organization. Nischarin is primarily a cytosolic protein, but clearly associates with alpha5beta1, as demonstrated by coimmunoprecipitation. Overexpression of Nischarin markedly reduces alpha5bet

SUBMITTER: Alahari SK 

PROVIDER: S-EPMC2190593 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature