Ontology highlight
ABSTRACT:
SUBMITTER: Rieger C
PROVIDER: S-EPMC2190707 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Nucleic acids research 20071210 22
Expression profiling of stem cells is challenging due to their small numbers and heterogeneity. The PCR colony (polony) approach has theoretical advantages as an assay for stem cells but has not been applied to small numbers of cells. An assay has been developed that is sensitive enough to detect mRNAs from small numbers of ES cells and from fractions of a single mouse blastocyst. Genes assayed include Oct3, Rex1, Nanog, Cdx2 and GLUT-1. The assay is highly sensitive so that multiple mRNAs from ...[more]