A comparison of microarray and MPSS technology platforms for expression analysis of Arabidopsis.

ABSTRACT:

Background

Several high-throughput technologies can measure in parallel the abundance of many mRNA transcripts within a sample. These include the widely-used microarray as well as the more recently developed methods based on sequence tag abundances such as the Massively Parallel Signature Sequencing (MPSS) technology. A comparison of microarray and MPSS technologies can help to establish the metrics for data comparisons across these technology platforms and determine some of the factors affecting the measurement of mRNA abundances using different platforms.

Results

We compared transcript abundance (gene expression) measurement data obtained using Affymetrix and Agilent microarrays with MPSS data. All three technologies were used to analyze the same set of mRNA samples; these

SUBMITTER: Chen J 

PROVIDER: S-EPMC2190774 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature