Treponema pallidum major sheath protein homologue Tpr K is a target of opsonic antibody and the protective immune response.

ABSTRACT: We have identified a family of genes that code for targets for opsonic antibody and protective immunity in T. pallidum subspecies pallidum using two different approaches, subtraction hybridization and differential immunologic screening of a T. pallidum genomic library. Both approaches led to the identification of a polymorphic multicopy gene family with predicted amino acid homology to the major sheath protein of Treponema denticola. One of the members of this gene family, tpr K, codes for a protein that is predicted to have a cleavable signal peptide and be located in the outer membrane of the bacterium. Reverse transcription polymerase chain reaction analysis of T. pallidum reveals that Tpr K is preferentially transcribed in the Nichols strain of T. pallidum. Antibodies directed to purif

SUBMITTER: Centurion-Lara A 

PROVIDER: S-EPMC2192927 | biostudies-literature | 1999 Feb

REPOSITORIES: biostudies-literature