A versatile approach to multiple gene RNA interference using microRNA-based short hairpin RNAs.

ABSTRACT:

Background

Effective and stable knockdown of multiple gene targets by RNA interference is often necessary to overcome isoform redundancy, but it remains a technical challenge when working with intractable cell systems.

Results

We have developed a flexible platform using RNA polymerase II promoter-driven expression of microRNA-like short hairpin RNAs which permits robust depletion of multiple target genes from a single transcript. Recombination-based subcloning permits expression of multi-shRNA transcripts from a comprehensive range of plasmid or viral vectors. Retroviral delivery of transcripts targeting isoforms of cAMP-dependent protein kinase in the RAW264.7 murine macrophage cell line emphasizes the utility of this approach and provides insight to cAMP-dependent transcri

SUBMITTER: Zhu X 

PROVIDER: S-EPMC2194719 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature