Disruption of Foxg1 expression by knock-in of cre recombinase: effects on the development of the mouse telencephalon.

ABSTRACT: The cre/loxP system is used routinely to manipulate gene expression in the mouse nervous system. In order to delete genes specifically from the telencephalon, the Foxg1-cre line was created previously by replacing the intron-less Foxg1 coding region with cre, resulting in a Foxg1 heterozygous mouse. As the telencephalon of heterozygous Foxg1 mice was reported to be normal, this genotype often has been used as the control in subsequent analyses. Here we describe substantial disruption of forebrain development of heterozygous mice in the Foxg1-cre line, maintained on the C57BL/6J background. High resolution magnetic resonance microscopy reveals a significant reduction in the volume of the neocortex, hippocampus and striatum. The alteration in the neocortex results, in part, from a decrease i

SUBMITTER: Eagleson KL 

PROVIDER: S-EPMC2194757 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature