Mapping the human plasma proteome by SCX-LC-IMS-MS.

ABSTRACT: The advent of on-line multidimensional liquid chromatography-mass spectrometry has significantly impacted proteomic analyses of complex biological fluids such as plasma. However, there is general agreement that additional advances to enhance the peak capacity of such platforms are required to enhance the accuracy and coverage of proteome maps of such fluids. Here, we describe the combination of strong-cation-exchange and reversed-phase liquid chromatographies with ion mobility and mass spectrometry as a means of characterizing the complex mixture of proteins associated with the human plasma proteome. The increase in separation capacity associated with inclusion of the ion mobility separation leads to generation of one of the most extensive proteome maps to date. The map is generated by ana

SUBMITTER: Liu X 

PROVIDER: S-EPMC2195767 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature