MAFA controls genes implicated in insulin biosynthesis and secretion.

ABSTRACT:

Aims/hypothesis

Effects of the transcription factor v-maf musculoaponeurotic fibrosarcoma oncogene homologue A (MAFA) on the regulation of beta cell gene expression and function were investigated.

Materials and methods

INS-1 stable cell lines permitting inducible up- or downregulation of this transcription factor were established.

Results

MAFA overproduction enhanced and its dominant-negative mutant (DN-MAFA) diminished binding of the factor to the insulin promoter, correlating with insulin mRNA levels and cellular protein content. Glucose-stimulated insulin secretion was facilitated by MAFA and blunted by DN-MAFA. This is partly due to alterations in glucokinase production, the glucose sensor of beta cells. In addition, the expression of important beta cell genes, e.

SUBMITTER: Wang H 

PROVIDER: S-EPMC2196442 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature