A novel genetic screen implicates Elm1 in the inactivation of the yeast transcription factor SBF.

ABSTRACT:

Background

Despite extensive large scale analyses of expression and protein-protein interactions (PPI) in the model organism Saccharomyces cerevisiae, over a thousand yeast genes remain uncharacterized. We have developed a novel strategy in yeast that directly combines genetics with proteomics in the same screen to assign function to proteins based on the observation of genetic perturbations of sentinel protein interactions (GePPI). As proof of principle of the GePPI screen, we applied it to identify proteins involved in the regulation of an important yeast cell cycle transcription factor, SBF that activates gene expression during G1 and S phase.

Methodology/principle findings

The principle of GePPI is that if a protein is involved in a pathway of interest, deletion of the c

SUBMITTER: Manderson EN 

PROVIDER: S-EPMC2198942 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature