Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells.

ABSTRACT: To identify genes expressed by a specific subset of dendritic cells found in vivo a polymerase chain reaction-based cDNA subtraction technique was applied to the recently described germinal center dendritic cells. A novel member of the disintegrin metalloproteinase family was cloned which comprises a not typical zinc-chelating catalytic site most similar to a bacterial metalloproteinase. Dendritic cell precursors or immature dendritic cells express no or low levels of the message. It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction. In situ hybridization showed distinct expression of this gene in the germinal center. This, together with the findings that certain disintegrin metalloproteinases regulate the activity of tumor necrosis

SUBMITTER: Mueller CG 

PROVIDER: S-EPMC2199019 | biostudies-literature | 1997 Aug

REPOSITORIES: biostudies-literature