Structure of PICK1 and other PDZ domains obtained with the help of self-binding C-terminal extensions.

ABSTRACT: PDZ domains are protein-protein interaction modules that generally bind to the C termini of their target proteins. The C-terminal four amino acids of a prospective binding partner of a PDZ domain are typically the determinants of binding specificity. In an effort to determine the structures of a number of PDZ domains we have included appropriate four residue extensions on the C termini of PDZ domain truncation mutants, designed for self-binding. Multiple truncations of each PDZ domain were generated. The four residue extensions, which represent known specificity sequences of the target PDZ domains and cover both class I and II motifs, form intermolecular contacts in the expected manner for the interactions of PDZ domains with protein C termini for both classes. We present the structures of

SUBMITTER: Elkins JM 

PROVIDER: S-EPMC2203335 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature