Dynamic transcription programs during ES cell differentiation towards mesoderm in serum versus serum-freeBMP4 culture.

ABSTRACT:

Background

Expression profiling of embryonic stem (ES) cell differentiation in the presence of serum has been performed previously. It remains unclear if transcriptional activation is dependent on complex growth factor mixtures in serum or whether this process is intrinsic to ES cells once the stem cell program has been inactivated. The aims of this study were to determine the transcriptional programs associated with the stem cell state and to characterize mesoderm differentiation between serum and serum-free culture.

Results

ES cells were differentiated as embryoid bodies in 10% FBS or serum-free media containing BMP4 (2 ng/ml), and expression profiled using 47 K Illumina(R) Sentrix arrays. Statistical methods were employed to define gene sets characteristic of stem cell, e

SUBMITTER: Bruce SJ 

PROVIDER: S-EPMC2204012 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature