Selective gene expression by postnatal electroporation during olfactory interneuron neurogenesis.

ABSTRACT: Neurogenesis persists in the olfactory system throughout life. The mechanisms of how new neurons are generated, how they integrate into circuits, and their role in coding remain mysteries. Here we report a technique that will greatly facilitate research into these questions. We found that electroporation can be used to robustly and selectively label progenitors in the Subventicular Zone. The approach was performed postnatally, without surgery, and with near 100% success rates. Labeling was found in all classes of interneurons in the olfactory bulb, persisted to adulthood and had no adverse effects. The broad utility of electroporation was demonstrated by encoding a calcium sensor and markers of intracellular organelles. The approach was found to be effective in wildtype and transgenic mice

SUBMITTER: Chesler AT 

PROVIDER: S-EPMC2204067 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature