Complete gene expression profiling of Saccharopolyspora erythraea using GeneChip DNA microarrays.

ABSTRACT:

Background

The Saccharopolyspora erythraea genome sequence, recently published, presents considerable divergence from those of streptomycetes in gene organization and function, confirming the remarkable potential of S. erythraea for producing many other secondary metabolites in addition to erythromycin. In order to investigate, at whole transcriptome level, how S. erythraea genes are modulated, a DNA microarray was specifically designed and constructed on the S. erythraea strain NRRL 2338 genome sequence, and the expression profiles of 6494 ORFs were monitored during growth in complex liquid medium.

Results

The transcriptional analysis identified a set of 404 genes, whose transcriptional signals vary during growth and characterize three distinct phases: a rapid growth until

SUBMITTER: Peano C 

PROVIDER: S-EPMC2206050 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature