Promiscuous gene expression patterns in single medullary thymic epithelial cells argue for a stochastic mechanism.

ABSTRACT: Promiscuous expression of tissue-restricted autoantigens in medullary thymic epithelial cells (mTECs) imposes central T cell tolerance. The molecular regulation of this unusual gene expression is not understood, in particular its delineation from cell lineage-specific gene expression control remains unclear. Here, we compared the expression profile of the casein gene locus in mTECs and mammary gland epithelial cells by single cell PCR. Mammary gland cells showed highly correlated intra- and interchromosomal coexpression of milk proteins (the casein genes, lactalbumin-alpha and whey acidic protein) and one of its transcriptional regulators (Elf5). In contrast, coexpression of these genes in mature CD80(hi) mTECs was rarely observed and no pattern of gene expression in individual mTECs was d

SUBMITTER: Derbinski J 

PROVIDER: S-EPMC2206592 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature