Correlation between CpG methylation profiles and hormone receptor status in breast cancers.

ABSTRACT:

Introduction

Aberrant DNA methylation has been found frequently in human breast cancers, associated with the loss of expression of a number of regulatory genes for growth and correlated to clinical outcomes. The present study was undertaken to determine whether methylation of a set of growth-suppressor genes would correlate to the expression of estrogen receptors (ERs) and progesterone receptors (PRs).

Methods

We used a pyrosequencing methylation analysis to study the methylation of 12 known growth-suppressor genes in 90 pairs of malignant/normal breast tissues. We also examined the expression of ERs and PRs in those specimens by immunohistochemistry. Mutations of p53 in tumor cells were detected by direct sequencing.

Results

Twelve tumor-suppressor genes: ARHI, RASSF

SUBMITTER: Feng W 

PROVIDER: S-EPMC2206733 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature