Theoretical study of the partial molar volume change associated with the pressure-induced structural transition of ubiquitin.

ABSTRACT: The partial molar volume (PMV) change associated with the pressure-induced structural transition of ubiquitin is analyzed by the three-dimensional reference interaction site model (3D-RISM) theory of molecular solvation. The theory predicts that the PMV decreases upon the structural transition, which is consistent with the experimental observation. The volume decomposition analysis demonstrates that the PMV reduction is primarily caused by the decrease in the volume of structural voids in the protein, which is partially canceled by the volume expansion due to the hydration effects. It is found from further analysis that the PMV reduction is ascribed substantially to the penetration of water molecules into a specific part of the protein. Based on the thermodynamic relation, this result impl

SUBMITTER: Imai T 

PROVIDER: S-EPMC2206979 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature