A large-scale proteomic analysis of human embryonic stem cells.

ABSTRACT:

Background

Much of our current knowledge of the molecular expression profile of human embryonic stem cells (hESCs) is based on transcriptional approaches. These analyses are only partly predictive of protein expression however, and do not shed light on post-translational regulation, leaving a large gap in our knowledge of the biology of pluripotent stem cells.

Results

Here we describe the use of two large-scale western blot assays to identify over 600 proteins expressed in undifferentiated hESCs, and highlight over 40 examples of multiple gel mobility variants, which are suspected protein isoforms and/or post-translational modifications. Twenty-two phosphorylation events in cell signaling molecules, as well as potential new markers of undifferentiated hESCs were also identif

SUBMITTER: Schulz TC 

PROVIDER: S-EPMC2211323 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature