A functional genomic yeast screen to identify pathogenic bacterial proteins.

ABSTRACT: Many bacterial pathogens promote infection and cause disease by directly injecting into host cells proteins that manipulate eukaryotic cellular processes. Identification of these translocated proteins is essential to understanding pathogenesis. Yet, their identification remains limited. This, in part, is due to their general sequence uniqueness, which confounds homology-based identification by comparative genomic methods. In addition, their absence often does not result in phenotypes in virulence assays limiting functional genetic screens. Translocated proteins have been observed to confer toxic phenotypes when expressed in the yeast Saccharomyces cerevisiae. This observation suggests that yeast growth inhibition can be used as an indicator of protein translocation in functional genomic sc

SUBMITTER: Slagowski NL 

PROVIDER: S-EPMC2211553 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature